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[目的]研究桐花树根际土壤真菌多样性及其发酵产物的抑菌活性。[方法]采用稀释涂布法分离桐花树根际土壤中的可培养真菌，基于ITS rDNA序列比对鉴定菌株，运用滤纸片琼脂扩散法和微孔板法评估根际土壤真菌发酵产物的抑菌活性。[结果]从桐花树根际土壤分离并鉴定的40株可培养真菌分属3纲5目7科11属，包括14株青霉菌属（Penicillium）菌株、13株篮状菌属（Talaromyces）菌株、5株曲霉属（Aspergillus）菌株、1株镰刀菌属（Fusarium）菌株、1株腐质霉属（Humicola）菌株、1株木霉属（Trichoderma）菌株、1株赛多孢霉属（Scedosporium）菌株、1株Westerdykella属菌株、1株Neoroussoella属菌株、1株Emericellopsis属菌株、1株Trichosporiella属菌株。22株根际土壤真菌的发酵产物至少对1种致病细菌有不同程度的抑制作用。[结论]桐花树根际土壤真菌群落有丰富的多样性，具有开发抗菌天然产物的价值。
Abstract：[Objective]To investigate the diversity of rhizosphere soil fungi of Aegiceras corniculatum and the antibacterial activity of their fermentation products.[Methods]The cultivable fungi in the rhizosphere soil of Aegiceras corniculatum were isolated by the dilution plating method. The fungi strains were identified based on ITS rDNA sequence alignment. The antibacterial activities of the fermentation products of these fungi strains were evaluated by the filter paper agar diffusion method and microplate method.[Results]A total of 40 cultivable fungal strains isolated and identified from the rhizosphere soil of Aegiceras corniculatum belonged to 3 classes, 5 orders, 7 families, and 11 genera. Specifically, there were 14 strains of Penicillium, 13 strains of Talaromyces, 5 strains of Aspergillus, 1 strain of Fusarium, 1 strain of Humicola, 1 strain of Trichoderma, 1 strain of Scedosporium, 1 strain of Westerdykella, 1 strain of Neoroussoella,1 strain of Emericellopsis, and 1 strain of Trichosporiella. The fermentation products of 22 strains exhibited inhibitory effects on at least one pathogenic bacterium.[Conclusion]The rhizosphere soil fungal community of Aegiceras corniculatum has rich diversity and possesses the potential value for developing antibacterial natural products.
[1]PALIT K,RATH S,CHATTERJEE S,et al.Microbial diversity and ecological interactions of microorganisms in the mangrove ecosystem:threats,vulnerability,and adaptations[J]. Environmental Science and Pollution Research,2022,29(22):32467-32512.
[2]JIA S L,CHI Z,LIU G L,et al.Fungi in mangrove ecosystems and their potential applications[J].Critical Reviews in Biotechnology,2020,40(6):852-864.
[3]ANCHEEVA E,DALETOS G,PROKSCH P.Lead compounds from mangrove-associated microorganisms[J].Marine Drugs,2018,16(9):319.
[4]JOB N,SARASAN M,PHILIP R.Mangrove-associated endomycota:diversity and functional significance as a source of novel drug leads[J].Archives of Microbiology,2023,205(11):349.
[5]WANG K W,WANG S W,WU B,et al.Bioactive natural compounds from the mangrove endophytic fungi[J].Mini Reviews in Medicinal Chemistry,2014,14(4):370-391.
[6]DESHMUKH S K,GUPTA M K,PRAKASH V,et al. Mangrove-associated fungi:a novel source of potential anticancer compounds[J].Journal of Fungi,2018,4(3):101.
[7]LUO Y Y,LUO X M,ZHANG T,et al.Anti-tumor secondary metabolites originating from fungi in the south China sea's mangrove ecosystem[J].Bioengineering,2022,9(12):776.
[8]CHEN S H,CAI R L,LIU Z M,et al.Secondary metabolites from mangrove-associated fungi:source,chemistry and bioactivities[J].Natural Product Reports,2022,39(3):560-595.
[9]XU J.Bioactive natural products derived from mangrove-associated microbes[J].RSC Advances,2015,5(2):841-892.
[10]CHEN Z,ZHANG J J,HUANG C Y,et al.Penicipyrrolizidinones A-C,three pyrrolizidinone alkaloids with unprecedented skeletons from the mangrove-derived fungus Penicillium sp.DM27[J].Phytochemistry,2025,229:114273.
[11]ZHENG Y Y,LIANG Z Y,SHEN N X,et al.New naphtho-γ-pyrones isolated from marine-derived fungus Penicillium sp.HK1-22 and their antimicrobial activities[J].Marine Drugs,2019,17(6):322.
[12]YANG S Q,LI X M,CHEN X D,et al.Three new α-pyrone derivatives from the soil-derived fungus Penicillium herquei MA-370[J].Natural Product Research,2024,38(17):2983-2988.
[13]CHEN M,SHEN N X,CHEN Z Q,et al.Penicilones A-D,anti-MRSA azaphilones from the marine-derived fungus Penicillium janthinellum HK1-6[J].Journal of Natural Products,2017,80(4):1081-1086.（下转第27页）
[14]CHEN M,ZHENG Y Y,CHEN Z Q,et al.NaBr-induced production of brominated azaphilones and related tricyclic polyketides by the marine-derived fungus Penicillium janthinellum HK1-6[J].Journal of Natural Products,2019,82(2):368-374.
[15]LIANG Z Y,SHEN N X,ZHENG Y Y,et al.Two new unsaturated fatty acids from the mangrove rhizosphere soil-derived fungus Penicillium javanicum HK1-22[J].Bioorganic Chemistry,2019,93:103331.
[16]MENG L H,LI X M,LIU Y,et al.Penicibilaenes A and B,sesquiterpenes with a tricyclo[6.3.1.0(1,5)]dodecane skeleton from the marine isolate of Penicillium bilaiae MA-267[J].Organic Letters,2014,16(23):6052-6055.
[17]夏丽娟，张焜，黄华容，等.5种红树根际土壤真菌和内生真菌的分离及抑菌活性的研究[J].中国农学通报，2014,30(4):259-263.
[18]郑智胜，黄卫红，陈小尘，等.泉州湾桐花树和老鼠簕根际土壤细菌和真菌群落特征比较[J].应用海洋学学报，2023,42(4):686-697.
[19]罗志宏，依旺的，邢楠楠，等.桐花树活性内生真菌筛选及其抗菌化学成分的研究[J].广西植物，2023,43(11):2033-2041.
[20]NICOLETTI R,BELLAVITA R,FALANGA A.The outstanding chemodiversity of marine-derived Talaromyces[J]. Biomolecules,2023,13(7):1021.
[21]LEI L R,GONG L Q,JIN M Y,et al.Research advances in the structures and biological activities of secondary metabolites from Talaromyces[J]. Frontiers in Microbiology,2022,13:984801.
[22]LIU S,SU M,SONG S J,et al. Marine-derived Penicillium species as producers of cytotoxic metabolites[J]. Marine Drugs,2017,15(10):329.
[23]DE CARVALHO A C,OGAWA C Y J,DE CAMILLIS R L,et al. Penicillium genus as a source for anti-leukemia compounds:an overview from 1984 to 2020[J].Leuk Lymphoma,2021,62(9):2079-2093.
[24]LI H H,FU Y Q,SONG F H,et al.Recent updates on the antimicrobial compounds from marine-derived Penicillium fungi[J].Chemistry&Biodiversity,2023,20(12):e202301278.
[25]TOGHUEO R M K,BOYOM F F.Endophytic Penicillium species and their agricultural,biotechnological,and pharmaceutical applications[J].3 Biotech,2020,10(3):107.
基本信息:
中图分类号:R915
引用信息:
[1]金鑫,滕全,覃雪莲,等.桐花树根际土壤真菌多样性和抗菌活性研究[J].广西中医药大学学报,2025,28(06):15-21+27.
基金信息:
广西自然科学基金项目（编号：2020GXNSFAA297163）
2025-11-15
2025-11-15